Distribution of mitochondria in reconstructed mouse oocytes.

Developmental competence of gametes reconstructed by germinal vesicle transplantation from fresh and cryopreserved bovine oocytes.

OBJECTIVE To evaluate the use of fresh or frozen bovine oocytes as an animal model for reconstructing artificial gametes by germinal vesicle transplantation (GVT), to study nucleocytoplasmic interaction and define clinical procedures for ooplasm donation in humans. DESIGN Prospective experimental study. SETTING University-based experimental laboratory. ANIMAL(S) Bovine oocytes from slaugh...

Domains of high-polarized and low-polarized mitochondria may occur in mouse and human oocytes and early embryos.

BACKGROUND The magnitude of the inner mitochondrial membrane potential (deltapsim) appears to influence the level of certain mitochondrial activities including regulation of ionic fluxes and ATP liberation, activities that are often compartmentalized or location dependent in cells. Recent evidence suggests that within cells, mitochondria can be heterogeneous with respect to deltapsim, and that ...

The Effect of Vitrification and in vitro Culture on the Adenosine Triphosphate Content and Mitochondrial Distribution of Mouse Pre-Implantation Embryos

Background: The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. Methods: The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...

P-66: The Effect of Vitrification on Distribution of ?9, ?v, ?1, ?3 Integrins Mouse Oocytes at GV, IVM-MII and OV- MII Stages

Mitochondrial Distribution and ATP Content of Vitrified, In vitro Matured Mouse Oocytes

BACKGROUND The objective of this study was to investigate the effect of vitrification and in vitro maturation on the mitochondrial distribution and ATP content of oocytes. METHODS The oocytes at Germinal Vesicle (GV) and Metaphase II (MII) stages were recovered from 6-8 week old NMRI strain female mice. The oocytes were divided into vitrified and non-vitrified groups. Vitrification was done b...